A set of transcripts may be specified to rsem-prepare-reference in one of two ways. The simplest approach is to supply a FASTA-formatted file of transcript sequences. For example, such a file could be obtained from a reference genome database, a de novo transcriptome assembler, or an EST database. Alternatively, using the --gtf option, a gene annotation file (in GTF format) and the full genome sequence (in FASTA format) may be supplied. For commonly-studied species, these files may be easily downloaded from databases such as the UCSC Genome Browser Database [26] and Ensembl [27]. If the quality of existing gene annotations is in question, one can use a reference-based RNA-Seq transcriptome assembler, such as Cufflinks [28], to provide an improved set of gene predictions in GTF format. When gene-level abundance estimates are desired, an additional file specifying which transcripts are from the same gene may be specified (via the --transcript-to-gene-map option), or, if a GTF file is provided, the "gene_id" attribute of each transcript may be used to determine gene membership. With either method of specifying transcripts, RSEM generates its own set of preprocessed transcript sequences for use by later steps. For poly(A) mRNA analysis, RSEM will append poly(A) tail sequences to reference transcripts to allow for more accurate read alignment (disabled with --no-polyA). The scripts for preparing the reference sequences need only be run once per reference transcriptome as the transcript sequences are preprocessed in a sample-independent manner.
RCI (Royal Caribbean International) is a subsidiary company and largest brand of RCG (Royal Caribbean Group, fka RCCL-Royal Caribbean Cruises Ltd). This Norwegian-American corporation is currently ranked the world's second-largest passenger shipping company (after Carnival Corporation/39% market share). However, RCG owns the world's biggest passenger ships. As of 2020, RCG controlled 24% of the global ship cruising market (fleet passenger capacity), had full ownership of 4 brands (RCI, Celebrity, Silversea, Azamara), 49% stake in Pullmantur (51% owned by Springwater Capital), 50% stake in TUI Cruises (50% owned by TUI AG), 19% stake in Wamos Air (fka Air Pullmantur).
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On August 30th, RCG signed a deal with SpaceX/Space Exploration Technologies Corporation (2002-founded by Elon Musk) for using Starlink's satellites to provide faster and low-latency Internet connections across the global fleet (Royal Caribbean International, Celebrity, and Silversea ships). Trials on the new Starlink Internet services were successfully conducted on Freedom OTS in June. Starlink Internet installations were scheduled to be completed fleetwide in 2023-Q1.
An additional global product is now available: the ASTER Water Body Dataset (ASTWBD). This raster product identifies all water bodies as either ocean, river, or lake. Each GDEM tile has a corresponding Water Body tile.The GDEM and ASTWBD are available for download from NASA Earthdata and Japan Space Systems.This ASTER product is available at no charge for any user pursuant to an agreement between METI and NASA. For more information about the GDEM, see the Validation Report: ASTER GDEM V2 Validation Summary Report.
Software Name: Global Mapper 13 32 bit / 64 BitFile name: global_mapper13_setup.rar (32 bit ), global_mapper13_setup_64Bit.exe (64 bit)Setup Full Size: 44 MB (x86), 37 MB (x64)Latest Version Release Added On: 9th Feb 2014
Alignment can be parallelized by distributing reads across concurrent search threads. Bowtie allows the user to specify a desired number of threads (option -p); Bowtie then launches the specified number of threads using the pthreads library. Bowtie threads synchronize with each other when fetching reads, outputting results, switching between indices, and performing various forms of global bookkeeping, such as marking a read as 'done'. Otherwise, threads are free to operate in parallel, substantially speeding up alignment on computers with multiple processor cores. The memory image of the index is shared by all threads, and so the footprint does not increase substantially when multiple threads are used. Table 4 shows performance results for running Bowtie v0.9.6 on the four-core server with one, two, and four threads.
USGS DOQ files are in the public domain, and can be used for any purpose without restriction. They are available for free download from the USGS, or from various state and regional data clearinghouses as well as from the geoCOMMUNITY site. Digital orthoimagery data at 1-foot and 1-meter spatial resolution, collected from multiple sources, are available for user-specified areas, and even higher resolution imagery (HRO) data sets for certain areas are available from the National Map Viewer site.
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